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General-purpose methods for this include Hydrophobic Interaction (HIC) and ion-exchange (IEX) chromatography. HIC is a good method for fractionating water-soluble proteins by differences in their hydrophobicity [ABOVE]. Unlike reversed-phase chromatography, it is a nondenaturing mode. However, hydrophobic proteins would be difficult to keep in solution in HIC.
Column Name : PolyPROPYL A™ Column
Compound Name: Water-Soluble Proteins
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