Hydrophilic Interaction Chromatography of phosphorothioates

This is an alternative to anion-exchange that can be used with volatile solvents. The following example consists of phosphorothioates with the same base sequence as the conventional oligonucleotide above. Peaks are broader, although it is unclear how much of this is due to:

a) The mode used;
b) The diastereomeric composition of phosphorothioates; and
c) The use of a 5-µm column for HILIC instead of 3-µm.

Column Name : PolyWAX LP™ Columns

Compound Name : Phosphorothioates