HPLC Separation of Vitamin E and Vitamin E Acetate on Zodiac HST B Column

The Zodiac HST B column effectively separates Vitamin E and Vitamin E acetate, providing a reversed elution order compared to a traditional C8 column. This unique selectivity arises from an embedded basic functional group in the stationary phase, which introduces additional polar interactions during the separation process. The method delivers excellent peak shapes using a mass spectrometry-compatible mobile phase composed of water and acetonitrile (MeCN), with UV detection at 205 nm.