HPLC Separation of Pyrilamine, Trimipramine, Pindolol Using Hydrogen Bonding Mode

Many pharmaceutical compounds contain a mix of small hydrophobic and hydrophilic components. While conventional techniques such as reversed-phase chromatography, cation-exchange, and HILIC are often employed for their analysis, an alternative approach based on hydrogen-bonding interactions offers distinct advantages.

Using the Zodiac HST HB column, separation is achieved through hydrogen bonding between analytes and the stationary phase. This mechanism provides unique selectivity, excellent peak shape, and precise retention control.

The method is fully compatible with ELSD, LC/MS, and preparative chromatography, making it versatile for both analytical and scale-up applications. Additionally, this approach can be extended to the analysis of a broad range of drug molecules.