HPLC Separation of Pyridine-Based Organic Acids Using Zodiac HST OG Column

This method demonstrates the separation of pyridine-containing organic acids — including pyridinedicarboxylic acid, picolinic acid, isonicotinic acid, and nicotinic acid — using a Zodiac HST OG column. The separation utilizes a mobile phase of 95% acetonitrile with 0.1% trifluoroacetic acid (TFA), at a flow rate of 1.0 mL/min, and detection by ELSD. The method leverages both hydrophobic and ion-exclusion interactions to achieve efficient resolution of structurally similar acidic compounds within 10 minutes.