HPLC Separation of Pseudoephedrine, Norephedrine, Phenylephrine, and Norphenylephrine Using Hydrogen Bonding Mode

Neurotransmitters are polar, basic compounds that pose a challenge for traditional HPLC due to their high hydrophilicity. Common techniques for their analysis include reversed-phase chromatography with ion-pairing reagents, ion chromatography, HILIC, and mixed-mode chromatography.

An innovative approach has been developed using the Zodiac HST HB column, which separates analytes based on hydrogen-bond interactions. This technique provides unique and complementary selectivity compared to conventional methods, offering an effective alternative for analyzing polar basic compounds like neurotransmitters.

The method is fully compatible with ELSD, LC/MS, and preparative chromatography, making it suitable for a wide range of analytical and preparative applications.