HPLC Separation of Mixture of DMF and DMSO on Zodiac HST HB Column

Dimethylformamide (DMF) and Dimethyl sulfoxide (DMSO) are widely used organic solvents. Both are miscible with water and most organic solvents, and can dissolve a wide range of polar and nonpolar compounds.

These highly polar compounds can be effectively retained and separated under anhydrous (water-free) HPLC conditions using a Zodiac HST HB column, which leverages hydrogen-bonding as the primary separation mechanism. The method employs a gradient elution using acetonitrile (ACN) and methanol (MeOH) without the need for a buffer, enabling a simple and robust approach. Detection is performed using UV at 220 nm.