HPLC Separation of Isopropylamine and Glyphosate using Zodiac HST B Column

Isopropylamine and glyphosate are commonly associated as components in herbicide formulations, with glyphosate being the active ingredient and isopropylamine acting as a counterion in salt form. Due to their high polarity and distinct chemical properties, separating these compounds requires a specialized approach. Utilizing the Zodiac HST B mixed-mode reverse-phase column, both isopropylamine and glyphosate can be effectively retained and separated. This method ensures clear resolution of the analytes and is well-suited for quality control and regulatory testing in agrochemical formulations.