HPLC Separation of Drugs in Zodiac HST TH Column

The Zodiac HST TH column can be effectively used for the analysis of both hydrophobic and hydrophilic basic and acidic drugs, along with their corresponding counter-ions. This mixed-mode chromatographic approach is well-suited for analyzing a wide range of pharmaceutical formulations, including cold and cough medications, pain relievers, and other prescription or over-the-counter drugs.

Within the pH range of 3–5, the column exhibits cation-exchange, anion-exchange, and hydrophobic interactions, enabling comprehensive separation of complex drug compositions. Selectivity in this method is finely controlled by adjusting the acetonitrile content, buffer concentration, and buffer pH. The method is robust, reproducible, and applicable as a universal HPLC approach for the analysis of diverse drug molecules and formulations.