HPLC MS Method for Analysis of Berberine on Zodiac HST B Column

Home HPLC MS Method for Analysis of Berberine on Zodiac HST B Column

Description

Berberine is a natural alkaloid found in plants like Berberis, Coptis chinensis, and Hydrastis canadensis. It exhibits antimicrobial, anti-inflammatory, antioxidant, and potential antidiabetic and anticancer properties.

Berberine can be retained and analyzed using a Zodiac HST B mixed-mode column with an isocratic mobile phase of water, acetonitrile (MeCN), and ammonium formate buffer. Detection is performed using LC-MS at m/z 336 (positive mode).

Condition

 

 

 

 

 

 

  1. Berberine

Column Name : Zodiac HST B

Compound Name : Berberine

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