HPLC MS Analysis of Procaine on Zodiac HST- OG2 Column Copy

Procaine is a local anesthetic from the class of amino esters, commonly used to numb specific areas of the body during medical procedures or minor surgeries. It works by blocking nerve signals, causing temporary loss of sensation in the treated area. Procaine is frequently used in dental procedures, minor skin surgeries, and as a component in pain relief formulations. It is often combined with other substances, such as benzyl alcohol, to improve its stability and effectiveness. Procaine can be retained and analyzed using a ZODIAC HST OG2 mixed-mode stationary phase column. The analysis employs an isocratic method with a mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is performed using UV at 294 nm.