HPLC Method of Ofloxacin in Ofloxacin 0.3 % Ear Drops on Zodiac HST RP Column

Ofloxacin, an antibiotic used to treat eye and ear infections, can be analyzed using the Zodiac HST RP column. Retention is achieved with a water-MeCN gradient mobile phase containing a sulfuric acid buffer. Detection is performed at 253 nm using UV, providing a reliable method for quantifying Ofloxacin.