HPLC Method for Separation of S-Nitroso-L-glutathione and Glutathione oxidized on Zodiac HST OG Column

GSNO and GSSG are derivatives of glutathione, a tripeptide composed of glutamic acid, cysteine, and glycine, essential for maintaining cellular redox homeostasis.

• S-Nitroso-L-Glutathione (GSNO): Acts as a nitric oxide (NO) donor, playing a key role in cell signaling and protein S-nitrosylation.

• Oxidized Glutathione (GSSG): Formed by the disulfide linkage of two glutathione (GSH) molecules, typically under oxidative stress, and reduced back to GSH by glutathione reductase.

Chromatographic Analysis:

GSNO and GSSG can be retained, separated, and analyzed using a Zodiac HST OG reverse-phase column (4.6 × 150 mm, 5 µm, 100 Å) with a mobile phase containing water, acetonitrile (MeCN), and sulfuric acid as a buffer.