HPLC Method for Separation of Michler’s ketone and Crystal Violet on Zodiac HST OG Column

Michler’s ketone (4,4′-bis(dimethylamino)benzophenone) is an essential intermediate in dye synthesis, contributing to the production of Malachite Green, Methyl Violet, and Crystal Violet. It consists of two dimethylaniline units linked by a carbonyl group, enhancing its electron-donating properties for vibrant dye formation.

Chromatographic Analysis:

Michler’s ketone and Crystal Violet can be separated, retained, and analyzed on a Zodiac HST OG mixed-mode stationary phase column using an isocratic mobile phase of water, acetonitrile (MeCN), and sulfuric acid (H₂SO₄) as a buffer. Detection is performed in the UV-Vis range at 540, 590, and 200 nm.