HPLC Method for Separation and Analysis of Labetalol Stereoisomers in Pharmaceutical Formulations Using Zodiac HST B Column

Labetalol is commonly prescribed for the treatment of hypertension. It belongs to the beta-blocker class of medications and functions by inhibiting the effects of endogenous catecholamines, such as epinephrine, on the heart and blood vessels. This action helps to lower heart rate, reduce blood pressure, and decrease myocardial workload.

Labetalol and its stereoisomers can be effectively retained, separated, and analyzed using the Zodiac HST B mixed-mode stationary phase column. The method utilizes an isocratic mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as the buffering system. Detection is performed using UV at 230 nm, making the method suitable for routine pharmaceutical analysis.