HPLC Method for Determination of Hydroxyzine in Tablets on Zodiac HST-SB Column

Hydroxyzine is a first-generation antihistamine used to treat itching, hives, anxiety, and tension by blocking histamine receptors. It has both sedative and anxiolytic properties and is available in oral and injectable forms. Due to its drowsiness-inducing effects, caution is advised in activities requiring alertness. Hydroxyzine is prescription-only, with dosing guided by a healthcare provider.

The compound can be effectively analyzed using the Zodiac HST SB mixed-mode HPLC column, which utilizes hydrophobic and anion-exchange interactions. The isocratic method employs a mobile phase of water, acetonitrile (MeCN), and sulfuric acid, with UV detection at 232 nm.