HPLC Method for Analyzing Aminopyrazole on a Zodiac HST A Column

Aminopyrazole is a hydrophilic, basic compound commonly used as a building block in drug manufacturing. It can be retained on Zodiac bimodal HPLC columns through a combination of weak reversed-phase and strong cation-exchange mechanisms. Retention time is primarily influenced by cation-exchange interactions and the ion concentration in the mobile phase. A comparison of Zodiac HST A, Zodiac HST OG, and Zodiac HST OG2 highlights the relative retention strength of each column for basic compounds.