HPLC Method for Analysis of Thiourea on Zodiac HST P1 Column

Thiourea is a multifunctional chemical compound with the molecular formula (NH₂)₂CS. It shares structural similarity with urea, with the primary distinction being the substitution of the oxygen atom by a sulfur atom. In its pure form, thiourea appears as a white crystalline solid.

This compound finds broad usage across various fields. In organic synthesis, thiourea acts as an essential reagent. It is also used in the toning of silver-gelatin photographic prints, in hair care formulations and bleaching products, and serves as a radioprotective agent in certain cancer therapies.

In the field of agriculture, thiourea functions as a soil treatment additive, supporting seed germination and enhancing both flowering and fruit development.

For chromatographic analysis, thiourea can be effectively retained and detected using a Zodiac HST P1 mixed-mode column and a mobile phase composed of water and acetonitrile (MeCN), without the use of a buffer. UV detection is typically performed at 200 nm.

The limit of detection (LOD) for this analytical setup—comprising the Zodiac HST P1 column, method conditions, and thiourea as the analyte—has been evaluated. It is important to note that the LOD can vary between laboratories, even when similar analytical techniques are applied.