HPLC Method for Analysis of Taurine on Zodiac HST RP Column

Taurine (2-aminoethanesulfonic acid) is a zwitterionic organic compound and a non-essential, sulfur-containing amino acid. It plays a critical role as a neurotransmitter alongside glycine and gamma-aminobutyric acid (GABA). Taurine is also a component of chenodeoxycholic acid, one of the most abundant bile acids, where it aids in the emulsification of dietary fats to support digestion. Due to its high polarity and zwitterionic nature, taurine presents a challenge for traditional reversed-phase chromatography.

Two effective methods have been developed for taurine analysis using mixed-mode chromatography. Taurine is retained on the Zodiac HST A column via a HILIC cation-exchange mechanism, and on the Zodiac HST RP column via a HILIC anion-exchange mechanism. These complementary approaches enable fast, robust, and selective quantification of taurine in a wide range of matrices, including energy drinks, food, and pharmaceutical formulations.

As taurine lacks strong UV absorbance, detection must be performed using ELSD or LC/MS methods to ensure reliable analysis.