Description
Ampicillin, a β-lactam antibiotic, is efficiently separated using the Zodiac HST OG2 mixed-mode column, which offers both reverse-phase and ion-exchange interactions. A gradient of acetonitrile and phosphoric acid (H₃PO₄) enables excellent resolution and peak shape. The acidic buffer suppresses ionic tailing and enhances analyte elution . UV detection at 270 nm provides sensitive and selective monitoring of Ampicillin, making this method ideal for pharmaceutical analysis in QC and formulation labs .
Condition
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