HPLC Method for Analysis of Methylprednisolone on Zodiac HST B Column

Methylprednisolone is a synthetic corticosteroid known for its potent anti-inflammatory and immunosuppressive effects. As a derivative of prednisolone and part of the glucocorticoid class, it is widely used to manage various medical conditions involving inflammation and immune system overactivity.

Methylprednisolone can be effectively retained and analyzed using a Zodiac HST B mixed-mode stationary phase column. The analytical method employs an isocratic mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is performed using UV at 245 nm, ensuring precise and reliable quantification.