HPLC Method for Analysis of Methyl cholate on Zodiac HST B Column

Methyl cholate is a derivative of cholic acid, a primary bile acid involved in the digestion and absorption of dietary fats. Formed by methylation of cholic acid, methyl cholate belongs to the broader class of bile acids, which are synthesized in the liver and released into the small intestine to aid in fat emulsification and the absorption of fat-soluble vitamins.

In addition to their digestive roles, bile acids—including methyl cholate—are involved in regulating cholesterol metabolism and function as signaling molecules in various physiological pathways.

Methyl cholate can be retained and analyzed using a Zodiac HST B mixed-mode stationary phase column. The analysis is carried out using an isocratic mobile phase composed of water, acetonitrile (MeCN), and sulfuric acid, with UV detection at 192 nm.