HPLC Method for Analysis of Hymexazol (3-Hydroxy-5-methylisoxazole) on Zodiac HST B Column

3-Hydroxy-5-methylisoxazole can be effectively retained and analyzed using a Zodiac HST B mixed-mode stationary phase column. The method utilizes an isocratic mobile phase composed of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is carried out using UV at 222 nm, providing reliable and accurate quantification of the compound.