HPLC Method for Analysis of Clonidine on Zodiac HST B Column

Clonidine is a medication primarily used for the treatment of high blood pressure (hypertension), particularly in cases where other antihypertensive agents have proven ineffective or poorly tolerated. It is available in various formulations, including oral tablets, transdermal patches, and oral suspensions.

Clonidine can be retained and analyzed using the Zodiac HST B mixed-mode stationary phase column. The method employs a gradient elution with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is performed using UV at 290 nm, making this a reliable and robust method for the analysis of clonidine in pharmaceutical dosage forms.