HPLC Method for Analysis of Bupivacaine on Zodiac HST OG2 Column

Description

Bupivacaine is a local anesthetic from the amide class, used to block nerve impulses and provide regional pain relief. It’s commonly administered via injection or topical application for surgical, dental, and obstetric procedures, as well as for managing chronic pain.

With a rapid onset (1–4 minutes) and long-lasting effects, Bupivacaine is suitable for procedures requiring extended anesthesia. However, improper administration or systemic absorption can lead to serious side effects, including cardiovascular and neurological complications, making professional oversight essential.

Bupivacaine can be effectively analyzed using the Zodiac HST OG2, 3.2 × 100 mm, 5 µm, 100 Å reverse-phase column. The method uses a mobile phase of water, acetonitrile (MeCN), and phosphoric acid as a buffer, with detection by UV at 270 nm.

Condition

1. Bupivacaine