HPLC Method for Analysis of Benztropine mesylate on Zodiac HST-SB Column

Benztropine is a pharmaceutical agent used in the treatment of Parkinson’s disease, acting as an acetylcholine antagonist to help reduce muscle stiffness. The compound can be effectively retained in HPLC using the Zodiac HST SB mixed-mode column, which leverages both hydrophobic and anion-exchange interactions. The isocratic method utilizes a mobile phase composed of acetonitrile (ACN) and water with a sulfuric acid buffer, and detection is performed using UV at 220 nm.