HPLC Method for Analysis of Amphetamine on Zodiac HST- OG2 Column

Description

Amphetamine, a central nervous system stimulant, is commonly prescribed for ADHD, narcolepsy, and obesity.

🧪 Method Overview: This RP-HPLC method utilizes a mobile phase composed of acetonitrile (MeCN), water, and phosphoric acid for sharp peak resolution and consistent retention.

⚙️ MS Compatibility: For LC-MS applications, formic acid is recommended instead of phosphoric acid to ensure volatility and MS detection compatibility.

⏱️ UPLC Adaptability: The method is compatible with 3 µm particle columns for faster analysis and is scalable to prep-scale for impurity isolation.

🧬 Applications: Ideal for pharmacokinetic studies and routine QC of amphetamine and its related compounds.

Condition

1. Amphetamine