HPLC ELSD Method for Analysis Spermidine on Zodiac HST TH Column

Spermidine is a polyamine compound present in all living cells. As a biogenic amine, it plays key roles in various cellular functions.

Biological Roles:

• Cell Growth and Proliferation: Spermidine contributes to the regulation of cellular growth and division.

• DNA Stability: It interacts with nucleic acids, helping maintain the structural integrity of DNA and RNA.

• Antioxidant Properties: Studies suggest spermidine may offer protection against oxidative stress due to its antioxidant characteristics.

• Autophagy Induction: Spermidine is known to promote autophagy, a cellular mechanism for degrading and recycling cellular components.

Natural Sources:

• Spermidine is naturally present in foods such as wheat germ, soybeans, mushrooms, and aged cheese.

• The body also synthesizes spermidine from precursor polyamines.

Research and Health Implications:

• Spermidine has attracted attention for its potential to support healthy aging and mitigate age-related diseases.

• Some research indicates that spermidine supplementation may benefit cardiovascular health, provide neuroprotective effects, and enhance longevity.

Supplementation:

• Spermidine is available in dietary supplement form and is taken by some individuals seeking its possible health advantages.

• However, further research is needed to determine safe and effective long-term dosage.

Analytical Method:

Spermidine can be effectively retained and analyzed using a Zodiac HST TH mixed-mode stationary phase column. The analysis utilizes an isocratic method with a straightforward mobile phase composed of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is carried out using evaporative light scattering detection (ELSD).