HPLC ELSD Method for Analysis Putrescine on Zodiac HST TH Column

Putrescine is a naturally occurring biogenic amine and a key member of the polyamine family, involved in cell growth, proliferation, and biosynthesis of other biomolecules. It is formed through the decarboxylation of ornithine and is found in various biological systems. For analytical purposes, putrescine can be separated using the Zodiac HST TH mixed-mode column, which combines ion-exchange and reversed-phase mechanisms. The method utilizes an isocratic mobile phase of water, acetonitrile, and ammonium formate, with ELSD detection, enabling reliable analysis in complex biological or industrial matrices.