Description
Serotonin, a key neurotransmitter with pharmaceutical significance, can be effectively retained and analyzed using the Zodiac HST OG2 mixed-mode column. The analysis is performed with an isocratic mobile phase of acetonitrile (MeCN) and water (30:70), buffered with 20 mM ammonium formate (AmFm) at pH 3.0. This MS-compatible method offers sharp peak shapes and reliable separation, with UV detection at 275 nm.
Condition
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