HPLC Determination of Mycophenolic Acid on Zodiac HST-SB Column

Mycophenolic acid, when used in combination with other medications, serves as an immunosuppressant to help prevent organ rejection following transplantation. The compound can be effectively retained using the Zodiac HST SB mixed-mode HPLC column, which incorporates both hydrophobic and anion-exchange interactions. The analytical method is isocratic, employing a mobile phase consisting of acetonitrile (ACN) and water with a phosphoric acid (H₃PO₄) buffer. Detection is carried out using UV at 226 nm.