HPLC Determination of Mycophenolate Mofetil on Zodiac HST-SB Column

Mycophenolate mofetil is an immunosuppressant commonly used alongside other medications to prevent organ rejection following transplantation. It can be effectively retained using the Zodiac HST SB mixed-mode column, which combines hydrophobic and anion-exchange properties. The HPLC method employs a gradient mobile phase of acetonitrile (ACN) and water with a phosphoric acid (H₃PO₄) buffer, with UV detection at 250 nm.