HPLC Determination of Coenzyme Q10 and Ubiquinol on Zodiac HST RP Column

Coenzyme Q10 (ubiquinone) is a naturally occurring compound found in animals and commonly used as a dietary supplement. Its reduced form, ubiquinol, shares a similar molecular structure, making the two challenging to separate using traditional reverse-phase HPLC methods. However, effective isocratic separation of CoQ10 and ubiquinol can be achieved using a Zodiac HST RP mixed-mode column with a mobile phase composed of acetonitrile (ACN) and water, eliminating the need for buffers. Detection can be performed using UV at 275 nm.