HPLC Determination of Azithromycin on Zodiac HST-SB Column

Azithromycin is a broad-spectrum antibiotic widely used to treat various bacterial infections. Listed as an essential medicine by the WHO and classified as critically important, it plays a key role in global healthcare. In conventional reversed-phase HPLC, azithromycin often shows significant peak tailing. This issue can be effectively minimized using the Zodiac HST SB mixed-mode column, combined with a simple, MS-compatible mobile phase consisting of water, acetonitrile (ACN), and ammonium formate (AmFm) buffer.