HPLC Analysis of Vitamin B

Zodiac HST C provides baseline resolution of B vitamins through a synergistic mechanism involving cation exchange, complex formation, and hydrophobic interactions. Vitamins B1 (thiamine), B2 (riboflavin), and B6 (pyridoxine) are effectively separated using a mobile phase composed of water, acetonitrile (MeCN or ACN), and triethylamine (TEA) phosphate, with UV detection at 280 nm.