HPLC Analysis of trans-Zeatin on Zodiac HST OG Column

trans-Zeatin is a cytokinin, a plant hormone that promotes cell division, growth, and delays aging, playing a vital role in plant development. It is one of the most active and commonly occurring forms of zeatin in plants.

It participates in key physiological processes such as seed germination, leaf formation, and root and shoot differentiation.

trans-Zeatin can be efficiently retained, separated, and analyzed using a Zodiac HST OG Column. The analysis follows an isocratic method with a water, acetonitrile (MeCN), and sulfuric acid mobile phase. Detection is performed using Vis at 200 nm for accurate results.