HPLC Analysis of Thiourea, Thiouracil (TU), Methylthiouracil (MTU) and Propylthiouracil (PTU) on Zodiac HST- P1 Column

For the analysis of Thiourea, Thiouracil (TU), Methylthiouracil (MTU), and Propylthiouracil (PTU) using a Zodiac HST P1 column, the following method can be used: These compounds can be separated and retained using an isocratic or gradient analytical method with a mobile phase consisting of water and acetonitrile (MeCN), with phosphoric acid as a buffer. The analysis can be detected using UV at 200 nm for optimal resolution and peak symmetry. This method provides accurate separation and quantification of these thiourea derivatives, ensuring reliable results in various applications such as pharmaceutical analysis and research