HPLC Analysis of Thiamine on Zodiac HST OG Column

Thiamine (Vitamin B1) is an essential nutrient that plays a key role in energy metabolism and nervous system function. It helps convert carbohydrates into energy and is vital for cell growth, development, and function.

Thiamine can be effectively retained, separated, and analyzed using a Zodiac HST OG Column with a mixed-mode stationary phase. The analysis follows an isocratic method with a water, acetonitrile (MeCN), and sulfuric acid mobile phase. Detection is performed using UV at 250 nm for accurate results.