HPLC Analysis Of Pyridylacetic Acids (2-Pyridylacetic acid, 3-Pyridylacetic acid, 4-Pyridylacetic acid) On Zodiac HST- S3 Column

Pyridylacetic acids, including 2-pyridylacetic acid, 3-pyridylacetic acid, and 4-pyridylacetic acid, are compounds derived from pyridine, commonly used in various chemical and pharmaceutical applications. The analysis of these acids can be effectively performed using High Performance Liquid Chromatography (HPLC) on a Zodiac HST-S3 column. The analysis employs a simple mobile phase composed of water and acetonitrile (MeCN), and detection is achieved using UV at a suitable wavelength. This method allows for the separation and quantification of the different pyridylacetic acid isomers with high precision.