HPLC Analysis of Opioid Alkaloids Using Zodiac HST OG Column

Uses Zodiac HST OG column to effectively separate the opioid compounds codeine, oxycodone, and hydrocodone based on hydrophobic and ion-exchange interactions. Using a mobile phase of 30% acetonitrile with 0.3% sulfuric acid and UV detection at 210 nm, the analytes are clearly resolved within 8 minutes. This technique is ideal for pharmaceutical analysis, forensic testing, and quality control of opioid-containing formulations.