HPLC Analysis of Lactic Acid and Its Oligomeric Forms

Description

Lactic acid (milk acid) is a naturally occurring organic acid that plays a vital role in various biochemical processes. It contains both carboxylic and hydroxyl groups, which can undergo self-condensation over time to form dimers, trimers, and higher oligomers.

Due to its high polarity, lactic acid exhibits poor retention on traditional reversed-phase columns. However, using the Zodiac HST B2 mixed-mode anion-exchange column, lactic acid and its oligomers can be effectively separated through a combination of weak reversed-phase and anion-exchange interactions.

Lactic acid oligomers can be hydrolyzed back to monomeric lactic acid, allowing for comprehensive analysis of both the acid and its oligomeric forms. The Zodiac HST B2 column is suitable for analyzing lactic acid as a standalone compound or within complex mixtures.

Detection can be performed using low-wavelength UV, ELSD, or LC/MS. When using ELSD, CAD, or LC/MS, the phosphoric acid typically used in the mobile phase should be replaced with a detection-compatible buffer, such as ammonium formate or trifluoroacetic acid (TFA), to ensure optimal sensitivity and performance.

Condition

1. Lactic Acid