HPLC Analysis of Ethephon in Hydrogen-Bonding Mode on Zodiac HST HB Column

Ethephon is a key compound used in the agricultural industry, known for its ability to release ethylene upon metabolism in plants. While corrosive in nature, it rapidly converts to ethylene, making it effective for regulating plant growth.

Ethephon can be retained and separated using the Zodiac HST HB column, which operates on a hydrogen-bonding separation mechanism. Retention is modulated by adjusting the methanol content in the mobile phase, which helps suppress excessive hydrogen bonding, allowing precise control over analyte retention.