HPLC Analysis Of Dopamine and Serotonin on Zodiac HST- OG2 Column

Dopamine and serotonin are key neurotransmitters with important pharmaceutical uses—dopamine as a cardiovascular stimulant and serotonin in antidepressant therapies. These compounds can be retained, separated, and analyzed using the Zodiac HST OG2 mixed-mode column with an isocratic mobile phase of water, acetonitrile (MeCN), and ammonium formate (AmFm) buffer. The method offers high-resolution UV detection at 280 nm with excellent peak symmetry.