HPLC Analysis of Dimethylthiourea on Zodiac HST- P1 Column

Dimethylthiourea (DMTU), with the chemical formula (CH3)2NCSNH2, is a compound used in research due to its properties as a hydrogen bond donor and acceptor. It is employed in reactions as a reducing agent and has applications in electrical conductivity and radiation absorption. For its analysis, the Zodiac HST P1 mixed-mode column can be used with a mobile phase consisting of water and Acetonitrile (MeCN), with no buffer. This simple mobile phase allows for effective retention and separation of DMTU. UV detection at 200 nm can be employed for accurate quantification. The limit of detection (LOD) may vary depending on the laboratory and instrument used. This method provides high resolution and efficient analysis of Dimethylthiourea.