HPLC Analysis of Dihydrotestestrone on Zodiac HST- OG Column

Dihydrotestosterone (DHT) is an androgen hormone derived from testosterone by the enzyme 5-alpha-reductase. It plays a vital role in the development of male characteristics such as facial and body hair growth, deepening of the voice, and muscle development. Elevated DHT levels are linked to conditions like androgenetic alopecia (male pattern baldness) and benign prostatic hyperplasia (enlarged prostate). 5alpha-Dihydrotestosterone can be retained, separated, and analyzed using a ZODIAC HST OG mixed-mode stationary phase column. The analysis utilizes an isocratic method with a mobile phase composed of water, acetonitrile (MeCN), and phosphoric acid as a buffer, with UV detection at 200 nm.