HPLC Analysis of Benzalkonium chloride on Zodiac HST- SB Column

Benzalkonium chloride is a quaternary ammonium compound and cationic surfactant used for its antimicrobial properties, effective against bacteria, viruses, and fungi by disrupting cell membranes. It is commonly found in healthcare settings for surface disinfection, ophthalmic solutions, nasal sprays, and as a preservative in personal care products like antiseptic wipes and hand sanitizers. The compound has different variants based on alkyl chain lengths, which influence its properties. Benzalkonium chloride can be retained and analyzed using a Zodiac HST SB column with a gradient method consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer, detected by UV and LC-MS positive mode.