Description
The Zodiac HST C column separates acetaminophen (paracetamol), phenylephrine, and diphenhydramine—common active ingredients found in over-the-counter cold and flu medications, including Tylenol products—using either an acetonitrile or phosphoric acid gradient.
The HPLC method employs a mobile phase consisting of water, acetonitrile (MeCN/ACN), and phosphoric acid, with detection performed at 210 nm using ultraviolet (UV) absorbance. This approach provides reliable separation and quantitation of these compounds in pharmaceutical formulations.
Condition
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