Glycopeptides

Selectivity in HILIC is the opposite of that of RPC. It is very sensitive to addition or deletion of a Ser- or carbohydrate residue, less sensitive to addition or deletion of a Leu- or Phe- residue. Thus, the two modes are complementary and are sometimes used in sequence to purify complex mixtures. An example is the assessment of the degree of glycation of γ-interferon. The tryptic fragments are resolved on a RPC column. The two glycopeptide peaks are collected and rerun via HILIC on aPolyHYDROXYETHYL A™ column. This resolves each peak into a library of glycopeptide peaks, each differing from its neighbor by one carbohydrate residue.

Application Name : Purification Of Variant Glycopeptides

Column Name : PolyHYDROXYETHYL A™ Columns

Analytes : Glycopeptides

RPC-HILIC: This separates peptides first by their hydrophobic and then by their hydrophilic residue differences. Capacity is less than with SCX, but either mode can be used with mobile phases compatible with MS.

Application Name : Isolation Of Glycopeptide By RPC & HILIC

Column Name : PolyHYDROXYETHYL A™ Columns

Analytes : Glycopeptides