Effect of pH on Separation of Glyphosate and Ethylphosphonic Acid

Glyphosate is a broad-spectrum herbicide commonly used for weed control, and its presence in water, fruits, and vegetables is closely regulated. Alkylphosphonic acids, which are degradation products of certain chemical warfare agents, are also subject to environmental monitoring by the Environmental Protection Agency (EPA). These compounds are highly polar and acidic in nature. A robust LC/MS-compatible method was developed for the analysis of glyphosate and ethylphosphonic acid, utilizing ion-exchange retention mechanisms. This method is suitable for detecting glyphosate and alkylphosphonic acids across a variety of sample matrices, including water and agricultural products.