Effect of pH on Retention of Basic Compounds on Zodiac HST C Column

This HPLC method demonstrates the separation of key components in cough medications, including acetaminophen, phenylephrine, and guaifenesin. The retention and elution order of these compounds can be adjusted by modifying the mobile phase composition, offering flexibility in method development.

The method is compatible with UV, ELSD, and LC/MS detection, making it suitable for the quantitation of active pharmaceutical ingredients in various formulations. The Zodiac HST C mixed-mode HPLC column is an excellent choice for analyzing cough medication components. Its unique stationary phase enables strong retention of basic compounds without the need for external ion-pairing reagents.

This approach has been validated by multiple pharmaceutical companies and is recognized for its robustness and reproducibility. It is well-suited for both research and development as well as production environments. Moreover, this method can be adapted as a general analytical strategy for separating basic compounds in complex mixtures.